Cell Culture & Cryopreservation Technician

Cell Culture & Cryopreservation Technician

Location:

Irvine, California

Job Type: Full-Time

About Us

We are an innovative regenerative medicine laboratory, tissue bank, and research facility dedicated to developing and processing advanced biologic therapies, including stem cell-based products. Our operations adhere to FDA regulations and emphasize quality, safety, and scientific excellence.

Position Overview

We are seeking a detail-oriented and highly skilled Cell Culture & Cryopreservation Technician with hands-on experience in stem cell culture and biobanking. The ideal candidate will have proven expertise in expanding, processing, and cryopreserving human stem cells within a regulated laboratory or tissue bank environment.

This role is essential to laboratory operations and requires precision, consistency, and strict adherence to aseptic techniques and Standard Operating Procedures (SOPs).

Key Responsibilities

• Perform aseptic cell culture techniques, including isolation, expansion, and maintenance of human cells (e.g., MSCs, SVF, and other primary cell types)
• Execute cryopreservation protocols, including controlled-rate freezing and storage in liquid nitrogen systems
• Thaw, assess viability, and recover cryopreserved cell samples
• Operate and maintain laboratory equipment (biosafety cabinets, incubators, centrifuges, controlled-rate freezers, LN₂ storage systems)
• Conduct cell counting, viability assays, and basic quality control (QC) testing
• Maintain accurate and compliant batch records, logs, and laboratory documentation
• Follow and contribute to the development and refinement of SOPs
• Ensure compliance with GTP/GMP guidelines and FDA regulations
• Manage inventory of biological samples, reagents, and consumables
• Maintain cleanroom standards and laboratory sterility
• Support QA/QC activities, audits, and regulatory inspections

Qualifications

Required

• Bachelor's degree (or higher) in Biology, Cell Biology, Biotechnology, or a related field
• Minimum of 2 years of hands-on cell culture experience (academic or industry)
• Direct experience with cryopreservation and thawing of cells
• Strong aseptic technique and sterile processing skills
• Experience working in a regulated laboratory environment (GTP, GMP, or GLP preferred)
• Experience with mesenchymal stem cells (MSCs), including adipose

Preferred

• Experience with umbilical-, or bone marrow-derived cells
• Familiarity with tissue banking procedures and workflows
• Experience operating controlled-rate freezers and liquid nitrogen storage systems
• Knowledge of FDA Good Tissue Practice (GTP) guidelines
• Experience with LIMS or electronic sample tracking systems
• Excellent documentation, organizational, and attention-to-detail skills

Compensation & Benefits

• Competitive salary (based on experience)
• Opportunities for growth within a cutting-edge regenerative medicine organization
• Health benefits (where applicable)
• Paid time off
• Ongoing training and professional development